| Index | Middleburg | Florida | National |
| Average Test Scores | 63% | 55% | 48% |
| Student/Teacher ratio | 17:1 | 17:1 | 16:1 |
| Total public schools | 7 | 3,388 | 79,221 |
| Total private schools | 3 | 1,640 | 24,874 |
| Total post-secondary schools | n/a | 466 | 7,963 |

| Index | Middleburg | Florida | National |
| Completed 8th grade | 80.4% | 84.0% | 84.3% |
| Completed high school | 79.0% | 83.0% | 83.0% |
| Completed some college | 43.8% | 58.0% | 59.4% |
| Completed associate degree | 19.7% | 37.4% | 38.5% |
| Completed bachelors | 11.1% | 27.9% | 30.3% |
| Completed masters | 4.4% | 10.0% | 11.5% |
| Completed professional degree | 0.6% | 3.1% | 3.3% |
| Completed doctorate | 0.4% | 1.1% | 1.3% |
There are a total of 7 public schools, 3 private schools and n/a post-secondary schools in Middleburg. Using the proficiency score averages, the average test scores for Middleburg, FL schools is 63%. The average student to teacher ratio is 17:1. In Middleburg, FL, an average of 80.4% of students have completed 8th grade and an average of 79.0% have completed high school.
| City | Population | Avg. Test Scores | National Avg. |
| Fruit Cove, FL | 33,956 | 84% | 75.5% |
| Fleming Island, FL | 31,515 | 72% | 50.5% |
| Lawtey, FL | 1,083 | 67% | 39.6% |
| Orange Park, FL | 8,984 | 64% | 34.1% |
| Green Cove Springs, FL | 8,488 | 49% | 2.6% |
| Middleburg, FL | 12,986 | 63% | 30.2% |
| City | Population | Avg. Test Scores | National Avg. |
| Athens, TX | 12,992 | 41% | 14.4% |
| Fords, NJ | 12,993 | 43% | 10.2% |
| Georgetown, GA | 12,996 | 17% | 64.1% |
| San Anselmo, CA | 13,003 | 76% | 59.1% |
| Fernandina Beach, FL | 13,006 | 70% | 44.8% |
| Middleburg, FL | 12,986 | 63% | 30.2% |